U2OS Cells stably expressing PAR2 Receptor, Calcium Biosensor & β-Arrestin Biosensor

Description U2OS Cells stably expressing PAR2 Receptor, Calcium Biosensor & β-Arrestin Biosensor are our multiplex NOMAD cells (MPX NOMAD). MPX NOMAD Cells are cell lines stably co-expressing tag-free GPCRs and two NOMAD Biosensors to monitor G-protein and  β-arrestin modulation. Each vial of MPX NOMAD PAR2 Receptor Cell Line contains U2OS cells stably expressing the following constructs: human PAR2 Receptor with no tag; Calcium NOMAD Biosensor (Green Fluorescence); β-arrestin NOMAD Biosensor (Red Fluorescence). MPX NOMAD PAR2 Receptor Cell Line allows to assay compounds analyzing both signalling pathways involving receptor activation; G protein by Ca2+ flux and  β-arrestin recruitment, hence allowing biased activity studies. When an agonist binds to PAR2 Receptor a G protein is activated which, in turn, triggers a cellular response mediated by calcium and a subsequent internalization mediated by  β-Arrestin. This leads to an increase of fluorescence intensity of both  β-Arrestin and Ca2+ biosensors. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.