U2OS cells stably expressing Beta-2 adrenergic Receptor and cAMP Biosensor

Description cAMP NOMAD Cells are cell lines stably co-expressing tag-free GPCRs and cAMP NOMAD Biosensor to monitor G-protein modulation by cAMP release. Each vial of cAMP NOMAD B2 adrenoceptor (ADRB2) Receptor Cell Line contains U2OS cells stably expressing the following constructs: (1) human B2 adrenoceptor (ARDB2) with no tag; (2) cAMP NOMAD Biosensor (Red Fluorescence). cAMP NOMAD B2 adrenoceptor (ARDB2) Cell Line allows to assay compounds analyzing the G-Protein signalling pathway by cAMP involving receptor activation. When an agonist binds to ADRB2 a G protein is activated which, in turn, triggers a cellular response mediated by cAMP. This leads to an increase of red fluorescence intensity of cAMP biosensor. So, the activity can be easily quantified on living cells in a dose-response manner by fluorescence emission in a microplate reader.